Two-year monitoring of enterovirus and rotavirus A in recreational freshwater from an island region, Pará State, northern Brazil

Enteric viruses are major causes of waterborne diseases and present in large quantities the stools infected individuals. Its viability environment lasts for months, favoring contamination water used consumption recreation. The study aimed to monitor monthly circulation enterovirus (EV) group A rotavirus (RVA) recreational freshwater from an island region as a bathhouse northern Brazil, January 2012 December 2013. viral RNA was obtained using guanidine isothiocyanate/silica after concentration by adsorption-elution method. molecular detection carried out semi nested-PCR amplicons were sequenced on automated sequencer. At least one these detected 40.4% (42/104) samples. RVA most frequent (n = 32; 30.8%) when compared EV 20; 19.2%). Co-circulation between both identified 9.6% 10). highest positivity found SP02 (46.1%). observed during high tides (57.7%; 60/104). Most samples characterized coxsackievirus (CV) A5 (85.7%, 12/14) others Sabin 1 poliovirus (14.2%, 2/14). positive genotyped G2, G3, G9, G12, P[8], P[4], P[6]. These 35.6% (37/104) with acceptable fecal coliform bacteria. results demonstrate surface intended recreation enteric Public Health concern even bacterial indicators within tolerated limit, factor that confirms need public policies sewage treatment before its release into bodies.